UV-Visible Spectrophotometer
UV-Visible Spectrophotometer
(Model : UV-1600 SHIMADZU)
Instrument Measurement Modes
Photometric
Measures the photometric value at a single wavelength or multiple (up to eight) wavelengths
Spectrum
Measures a sample spectrum using wavelength scanning.
Quantitation
Generates a calibration curve from the measurement of standards, and then calculates the concentrations of unknowns.
Kinetics
Measures absorbance changes as a function of time, and obtains the enzymatic activity values. The kinetics measurement method or the rate measurement method can be selected
Time Course
Measures changes over time in photometric values at a specified wavelength.
Biomethod
Quantifies DNA or protein concentrations
Food
This is an example of the analysis of food dyes. By using ultra-fast scan mode, the time needed for routine spectral checks can be shortened. The 350 nm to 900 nm region can be measured at 1 nm intervals in approx. 4 second
Pharmaceuticals and Life Sciences
This is an example of the analysis of ฮปDNA. Trace quantities (on the order of a few ยตL) can be measured by combining the instrument with Nano Stick and TrayCell ยฎ .
Using TrayCell, a calibration curve for 4 ยตL of ฮปDNA was obtained in the range between 25 ng/ยตL and 500 ng/ยตL
Chemistry
This is an example of the analysis of colored cellophane tape
Materials can be confirmed quantitatively by using LabSolutions
UV-Vis and color measurement software
Software available
1. UV Probe SHIMADZU
2. Lab Solutions SHIMADZU
Address for communication
Dr. H. Shankar
Materials Characterization Lab
Centre for Research and Development
KPR Institute of Engineering and Technology
Arasur, Coimbatore โ 641407
Email 1 : shankar.h@kpriet.ac.in
Email 2 : xrd@kpriet.ac.in
Landline : +91 422 2635600 (Internal โ 653)